clonetics™ cell systems  (Lonza)

Journal: Iranian Endodontic Journal

Article Title: Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study

doi: 10.22037/iej.v16i3.29449

Figure Lengend Snippet: Human periodontal ligament fibroblasts cultured in Dulbecco’s modified Eagle’s medium (200× magnification)

Article Snippet: Cell culture Human periodontal ligament fibroblasts (HPdLFs) (Cell System HPdLF Clonetics TM , Lonza Walkersville, Inc., Walkersville, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Merk Life Science, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS) (Merk Life Science, Darmstadt , Germany), 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 ° C in a humidified atmosphere of 5% CO 2 ( ).

Techniques: Cell Culture, Modification

Journal: Iranian Endodontic Journal

Article Title: Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study

doi: 10.22037/iej.v16i3.29449

Figure Lengend Snippet: Effects of fresh and set root canal sealers on the viability of HPdLFs assessed using flow cytometry. The cytotoxicity was determined based on a comparison between the proportions of apoptotic, necrotic and viable cell fractions, following the exposure of HPdLFs to the tested materials. The cumulative diagram shows the percentage of necrotic, early and late apoptotic, and viable cells (with standard deviation; SD) EN: Endomethasone N, RSEAL: RealSeal, RSA: Roeko Seal Automix, SP: Sealapex, HPdLFs: Human Periodontal Ligament Fibroblasts

Article Snippet: Cell culture Human periodontal ligament fibroblasts (HPdLFs) (Cell System HPdLF Clonetics TM , Lonza Walkersville, Inc., Walkersville, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Merk Life Science, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS) (Merk Life Science, Darmstadt , Germany), 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 ° C in a humidified atmosphere of 5% CO 2 ( ).

Techniques: Flow Cytometry, Comparison, Standard Deviation

Journal: Iranian Endodontic Journal

Article Title: Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study

doi: 10.22037/iej.v16i3.29449

Figure Lengend Snippet: Representative 2-dimenshional dot plots of the flow cytometry data derived from FITC-AnV and PI-stained HPdLFs after 24-hour exposure to fresh materials (a) and set materials (b). The dot plot represented the distribution of viable (lower left), early apoptotic (lower right), late apoptotic (upper right), and necrotic (upper left), respectively. FITC-AnV: Annexin V bound to fluorescein isothiocyanate, PI: Propidium iodide, HPdLFs: Human Periodontal Ligament Fibroblasts

Article Snippet: Cell culture Human periodontal ligament fibroblasts (HPdLFs) (Cell System HPdLF Clonetics TM , Lonza Walkersville, Inc., Walkersville, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Merk Life Science, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS) (Merk Life Science, Darmstadt , Germany), 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 ° C in a humidified atmosphere of 5% CO 2 ( ).

Techniques: Flow Cytometry, Derivative Assay, Staining

Journal: Iranian Endodontic Journal

Article Title: Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study

doi: 10.22037/iej.v16i3.29449

Figure Lengend Snippet: Effect of fresh and set materials on the mean percentage of intracellular ROS production in Human Periodontal Ligament Fibroblasts

Article Snippet: Cell culture Human periodontal ligament fibroblasts (HPdLFs) (Cell System HPdLF Clonetics TM , Lonza Walkersville, Inc., Walkersville, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Merk Life Science, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS) (Merk Life Science, Darmstadt , Germany), 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 ° C in a humidified atmosphere of 5% CO 2 ( ).

Techniques:

Journal: Iranian Endodontic Journal

Article Title: Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study

doi: 10.22037/iej.v16i3.29449

Figure Lengend Snippet: Representative histograms showing DCF distribution in HPdLFs after a 2-hour exposure to (a) fresh and (b) set materials. In most of the tested materials, a 2-population DCF fluorescence distribution was obtained. The number of cells in the right part of the histogram, in the P3 field, in comparison with the number of the control subpopulation, reflects the quantitative changes obtained as a result of the experiment, HPdLFs: Human Periodontal Ligament Fibroblasts, DCF: Dichlorodihydro fluorescence

Article Snippet: Cell culture Human periodontal ligament fibroblasts (HPdLFs) (Cell System HPdLF Clonetics TM , Lonza Walkersville, Inc., Walkersville, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Merk Life Science, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS) (Merk Life Science, Darmstadt , Germany), 100 μg/mL penicillin, and 100 μg/mL streptomycin at 37 ° C in a humidified atmosphere of 5% CO 2 ( ).

Techniques: Fluorescence, Comparison, Control